hCB1

Nomenclature

C57BL/6Smoc-Cnr1em1(hCB1)Smoc

Cat. NO.

NM-HU-225027

Strain State

Repository Live

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Gene Summary

Gene Symbol
Cnr1

Synonyms

CB1; CB-R; CB1R

NCBI ID

12801

MGI ID

104615

Ensembl ID

ENSMUSG00000044288

Pubmed

Cnr1

Human Ortholog

CNR1

Model Description

The endogenous mouse Cnr1 gene was replaced by human CB1 gene.

Validation Data

image.png

Fig1. Detection of CB1 expression in cerebellum by RT-PCR. 

Wild type: only one band at 373 bp with primers F1/R1(mCb1);

Homozygous: only one band at 209 bp with primers F2/R2(hCB1);

Abbr. M, DNA marker; HO, homozygous;  WT, wild type.image.png

Fig2. Detection of human CB1 expression in Brains, liver and kidney in hCB1 knockin mice by WB. 

Abbr. HO, homozygous; WT, wild type.

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

image.png

Fig3. Detection of human CB1 expression in brain (Hippocampus) in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type.

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

image.png

Fig4. Detection of human CB1 expression in CA1 in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.image.png

Fig5. Detection of human CB1 expression in CA3 in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.image.png

Fig6. Detection of human CB1 expression in dentate gyrus(DG) in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.image.png

Fig7. Detection of human CB1 expression in cerebellum in hCB1 knockin mice by IF. 

Brains were collected from wild-type mice and HO hCB1 mice, and analyzed by IF with anti-human CB1 antibody. 

Abbr. HO, homozygous; WT, wild type. 

Note. The anti-human CB1 Antibody cross-reacted with mouse CB1 and humanized CB1.

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