hMAPT

Nomenclature

C57BL/6Smoc-Mapttm1(hMAPT)Smoc

Cat. NO.

NM-HU-200116

Strain State

Repository Live

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Gene Summary

Gene Symbol
Mapt

Synonyms

Tau; Mtapt; AI413597; AW045860

NCBI ID

17762

MGI ID

97180

Ensembl ID

ENSMUSG00000018411

Pubmed

Mapt

Human Ortholog

MAPT

Model Description

The endogenous mouse Mapt gene was replaced by human MAPT gene. While hMAPT(2)(Stock No.NM-HU-200257) mice function similarly to hMAPT mice,for more detailed information please contact our technical advisor.

Validation Data

image.png

Fig.1 Detection of MAPT expression in cerebellum by RT-PCR. 

Wild type: only one band at 200 bp with primers F1/R1 (mMapt); 

Homozygous: only one band at 181 bp with primers F2/R2 (hMAPT); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig 2. Detection of mouse MAPT expression in Brain in hMAPT knockin mice by IHC. 

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody. 

Abbr. HO/HO, homozygous; +/+, wild type. 

Note. The anti-mouse MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

image.png

Fig 3. Detection of human MAPT expression in Brain in hMAPT knockin mice by IHC. 

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IHC with anti-human MAPT antibody. 

Abbr. HO/HO, homozygous; +/+, wild type. 

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

image.png

Fig 4. Detection of human MAPT expression in Brain in hMAPT knockin mice by IF. 

Brains were collected from wild-type mice (+/+) and homozygous hMAPT mice (HO), and analyzed by IF with anti-human MAPT antibody. 

Abbr. HO/HO, homozygous; +/+, wild type. 

Note. The anti-human MAPT Antibody cross-reacted with mouse MAPT and humanized MAPT.

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