Il1b-Luc-2A-EGFP

Nomenclature

C57BL/6Smoc-Il1bem1(Luc-eGFP)Smoc

Cat. NO.

NM-KI-00010

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol
IL1B

Synonyms

Il-1b; IL-1beta

NCBI ID

16176

MGI ID

96543

Ensembl ID

ENSMUSG00000027398

Pubmed

IL1B

Human Ortholog

IL1B

Model Description

A Luciferase-2A-EGFP-pA cassette was inserted at ATG site of mouse Il1b gene. The intrisic promoter drives the expression of reporter genes, which reflects the activity of Il1b.
Research Application：Inflammation and immunity,gene tracing

Validation Data

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Fig1. IL1β reporter gene expression in the mice was monitored through noninvasive molecular imaging. IL1 β  in the mouse body was activated through LPS  treatment.

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Fig2. Analysis of EGFP expresssion in PBMC by FACS. The homozygous KI mouse expresses EGFP in  F4/80+ macrophages in peripheral blood.

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Fig3. Analysis of EGFP expresssion in the spleen by FACS. The homozygous KI mouse expresses EGFP in F4/80+ macrophages in the spleen.

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Fig4. Analysis of EGFP expresssion in the liver by FACS. The homozygous KI mouse expresses EGFP in F4/80+ macrophages in the liver.


Publications

Acute pancreatic beta cell apoptosis by IL-1β is responsible for postburn hyperglycemia: Evidence from humans and mice
References：ELSEVIER

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