hCFB

Nomenclature

C57BL/6Smoc-Cfbtm1(hCFB)/Smoc

Cat. NO.

NM-HU-2000078

Strain State

Repository Live

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Gene Summary

Gene Symbol

Synonyms

B， Bf， C2， Fb， H2-Bf， AI195813， AI255840

NCBI ID

14962

MGI ID

105975

Ensembl ID

ENSMUSG00000090231

Pubmed

Human Ortholog

CFB

Model Description

The endogenous mouse Cfb gene was replaced by human CFB gene.
Research Application：Immunotherapy,drug screening

Validation Data

image.png

Fig1. Detection of CFB expression in liver by RT-PCR. 

Wild type: only one band at 441 bp with primers F1/R1(mCfb);

Homozygous: only one band at 442 bp with primers F2/R2(hCFB); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig2. Detection of human and mouse CFB expression in serum by ELISA (n=2). 

Abbr. HO, homozygous; WT, wild type.

Note: The mouse CFB ELISA kit cross-reacted with mCFB and humanized CFB.

image.png

Fig3. Detection of hCFB expression in serum by ELISA(n=3/group). 

Abbr. HO, homozygous; WT, wild type


image.png

Fig4. Detection of liver CFB mRNA remaining at Day 7 (A) and serum protein level (B) after single dose of test drug (n=3/group). (Data from a collaborator)

image.png

Fig5.Partial representative pictures of liver and kidney pathology in hCFB knockin mice. No obvious lesions were observed in hCFB knockin mice. 




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