hCD3EDG(BALB/c)
Nomenclature
BALB/c-Cd3etm1(hCD3E)Cd3dtm1(hCD3D)Cd3gtm1(hCD3G)/CbSmoc
Cat. NO.
NM-HU-220122
Strain State
Embryo cryopreservation
Gene Summary
Gene Symbol
CD3E
Gene Symbol
CD3D
Gene Symbol
CD3G
Model Description
Validation Data
Fig1. Detection of surface expression of human CD3E/CD3D on T cells in spleen in homozygous hCD3EDG (BALB/c) mice. (In collaboration with CrownBio.)
Fig2. Detection of surface expression of human CD3E/CD3G on T cells in blood in homozygous hCD3EDG (BALB/c) mice. (In collaboration with CrownBio.)
Fig3. Analysis of TCR signalling in hCD3EDG (BALB/c) mice upon CD3/CD28 activation in vitro. (In collaboration with CrownBio.)
Note: CFSE-dilution as a measure of mouse T cell proliferation.
CD3+ T cells were isolated in the splenocytes from WT BALB/c and hCD3EDG mice. Isolated T cells were labeled with µCFSE dye and stimulated with anti-mCD3 (5µg/mL) or anti-hCD3 (5µg/mL) plus soluble anti-mCD28 (50µg/mL) for in vitro culture for 72 hr. T cell proliferation was analyzed by flow cytometry for CFSE dilution.
You may also like
On Dec 16, 2018, Broad Institute and Shanghai Model Organisms Center Inc (SMOC) has entered into a non-exclusive license agreement under which Broad has granted SMOC worldwide rights to commercialize a service platform for genetically modified mouse models under Broad's intellectual property.
Learn moreAt GenoBioTX, we understand that the lengthy wait times for gene-modified mouse models can hinder your research progress. Traditional methods often require 6-9 months, leading to delays and increased costs. That’s why we’re thrilled to introduce our innovative service designed to streamline this process and deliver results faster.
Learn more
