Gpr65-IRES-Cre

Nomenclature

C57BL/6Smoc-Gpr65em1(IRES-iCre)Smoc

Cat. NO.

NM-KI-200135

Strain State

Embryo cryopreservation

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Gene Summary

Gene Symbol

Synonyms

Dig1， TDAG8， Gpcr25

NCBI ID

14744

MGI ID

108031

Ensembl ID

ENSMUSG00000021886

Pubmed

Human Ortholog

GPR65

Model Description

A IRES-iCre expression cassette was knocked into the Gpr65 gene stop codon site.
Research Application：Cre recombinase tool; The G-protein-coupled receptor(GPR65) neurons  innervate intestinal villi and gastric mucosa to sense nutrients. When crossed with a strain carrying a gene flanked by loxP sites, the flanked gene will be removed in cells expressing cre.

Validation Data

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Fig. 1 Cre-mediated recombination in the intestine of Gpr65Cre/+; Rosa26tdTomato/+ mouse. TdTomato(red) expression can be detected in the large intestine and small intestine derived from Gpr65Cre/+; Rosa26tdTomato/+ mouse.

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Fig. 2 Detection of tdTomato(red) in various tissues of Gpr65Cre/+; Rosa26tdTomato/+ mice. Cre mediated recombination can be detected in the pituitary gland, heart, liver, lung, stomach, salivary gland, kidney, pancreas, large intestine, skin, testis and epididymis. TdTomato can also be detected in individual cells of the thymus and spleen. Tdtomato expression can not be observed in brown fat. (For more detailed information please contact our technical advisor.)


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