Ptf1a-CreERT2

Nomenclature

C57BL/6Smoc-Ptf1aem1(CreERT2-WPRE-pA)Smoc

Cat. NO.

NM-KI-200118

Strain State

Repository Live

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Gene Summary

Gene Symbol
Ptf1a

Synonyms

PTF1p48; bHLHa29; PTF1-p48

NCBI ID

19213

MGI ID

1328312

Ensembl ID

ENSMUSG00000026735

Pubmed

Ptf1a

Human Ortholog

PTF1A

Model Description

A CreERT2-WPRE-pA expression cassette was knocked into the Ptf1a gene start codon site.
Research Application：These mice express cre recombinase from the Ptf1a locus. By mating the reporter mice with CreERT2-expressing mice, reporter gene expression can be detected in the pancreatic acinar cells of the Ptf1aCreERT2/+ ; Rosa26tdTomato/+ mouse. And the mice are useful for generating conditional mutations in pancreatic acinar cells. This strain may be useful for in the research of pancreatic cancer.

Validation Data

Ptf1a-CreERT2-1.png

Fig.1 CreERT2-mediated recombination in the pancreas of Ptf1aCreERT2/+ ; Rosa26tdTomato/+ mouse. 

TdTomato(red) expression can be detected in the pancreatic acinar cells of Ptf1aCreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment. 

Ptf1a-CreERT2-2.png

Fig.2 Detection of tdTomato (red) in various tissues of Ptf1aCreERT2/+; Rosa26tdTomato/+ mice after tamoxifen treatment. 

CreERT2-mediated recombination in the pancreatic acinar cells  can be induced by tamoxifen. Some leakiness were detected in the pancreas prior to tamoxifen exposure. Besides, tdtomato expression can be detected in a few cells of the brain, pituitary gland and stomach. Tdtomato exprssion can not be detected in thymus, heart, lung, liver, testis, kidney, intestine, brown fat, spleen, submandibular gland, and muscle. (For more detailed information please contact our technical advisor.)

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