hMASP2

Nomenclature

C57BL/6Smoc-Masp2em1(hMASP2)/Smoc

Cat. NO.

NM-HU-2000076

Strain State

Repository Live

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Gene Summary

Gene Symbol
Masp2

Synonyms

MAp19; MASP-2

NCBI ID

17175

MGI ID

1330832

Ensembl ID

ENSMUSG00000028979

Pubmed

Masp2

Human Ortholog

MASP2

Model Description

The endogenous mouse Masp2 gene was replaced by human MASP2 gene.
Research Application：Immunotherapy,drug screening

Validation Data

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Fig1. Detection of MASP2 expression in liver by RT-PCR. 

Wild type: only one band at 479 bp with primers F1/R1(mMasp2);

Homozygous: only one band at 338 bp with primers F2/R2(hMASP2); 

Abbr. M, DNA marker; HO, homozygous; WT, wild type.

image.png

Fig2. Detection of hMASP2 expression in liver in HO hMASP2 mice by western blot.

Abbr. HO, homozygous; WT, wild type.

Note: The anti-human MASP2 antibody cross-reacted with mouse MASP2 and humanized MASP2.

image.png

Fig3. Detection of hMASP2 expression in liver in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

image.png

Fig4. Detection of hMASP2 expression in kidney in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

image.png

Fig5. Detection of IgA expression in liver in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

image.png

Fig6. Detection of IgA expression in kidney in HO hMASP2 mice by IHC.

Abbr. HO, homozygous; WT, wild type.

image.png

Fig7. No obvious pathological features were found in liver in HO hMASP2 mice.

Abbr. HO, homozygous; WT, wild type.

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Fig8. No obvious pathological features were found in kidney in HO hMASP2 mice.

Abbr. HO, homozygous; WT, wild type.

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Fig9. Detection of mC3 expression in serum by ELISA. 

Abbr. HO, homozygous; WT, wild type.

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